iSEE

by iSEE

iSEE / iSEE

R/shiny interface for interactive visualization of data in SummarizedExperiment objects

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iSEE - The interactive SummarizedExperiment Explorer

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Bioconductor release status

| Branch | R CMD check | Last updated | |:----------------:|:----------------:|:------------:| | devel | Bioconductor-devel Build Status | | | release | Bioconductor-release Build Status | |

Overview

The iSEE package provides an interactive user interface for exploring data in objects derived from the

SummarizedExperiment
class. Particular focus is given to single-cell data stored in the
SingleCellExperiment
derived class. The user interface is implemented with RStudio's Shiny, with a multi-panel setup for ease of navigation.

This initiative was proposed at the European Bioconductor Meeting in Cambridge, 2017. Current contributors include:

Figure 1. _iSEE_ uses a customisable multi-panel layout.

Installation

iSEE can be easily installed from Bioconductor using

BiocManager::install()
:
if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install("iSEE")
# or also...
BiocManager::install("iSEE", dependencies = TRUE)

Setting

dependencies = TRUE
should ensure that all packages, including the ones in the
Suggests:
field of the
DESCRIPTION
, are installed - this can be essential if you want to reproduce the code in the vignette, for example.

Functionalities

Click to expand the list of features available in iSEE applications.

General

  • Multiple interactive plot types with selectable points.

  • Interactive tables with selectable rows.

  • Coloring of samples and features by metadata or expression data.

  • Zooming to a plot subregion.

  • Transmission of point selections between panels to highlight, color, or restrict data points in the receiving panel(s).

  • Lasso point selection to define complex shapes.

Sample-level visualization

The iSEE user interface currently contains the following components where each data point represents a single biological sample:

  • Reduced dimension plot: Scatter plot of reduced dimensionality data.

  • Column data plot: Adaptive plot of any one or two sample metadata. A scatter, violin, or square design is dynamically applied according to the continuous or discrete nature of the metadata.

  • Feature assay plot: Adaptive plot of expression data across samples for any two features or one feature against one sample metadata.

  • Column data table: Table of sample metadata.

Feature-level visualization

The iSEE user interface currently contains the following components where each data point represents a genomic feature:

  • Row data plot: Adaptive plot of any two feature metadata. A scatter, violin, or square design is dynamically applied according to the continuous or discrete nature of the metadata.

  • Sample assay plot: Adaptive plot of expression data across features for any two samples or one sample against one feature metadata.

  • Row data table: Table of feature metadata.

Integrated visualization

The iSEE user interface contains the following components that integrate sample and feature information:

  • Complex heatmap plot: Visualize multiple features across multiple samples annotated with sample metadata.

Custom panels

The iSEE API allows users to programmatically define their own plotting and table panels. See the section Extending iSEE further below.

Miscellaneous

  • The iSEE user interface continually tracks the code corresponding to all visible plotting panels. This code is rendered in a shinyAce text editor and can be copy-pasted into R scripts for customization and further use.

  • Speech recognition can be enabled to control the user interface using voice commands.

Want to try iSEE?

We set up instances of iSEE applications running on diverse types of datasets at those addresses:

  • http://shiny.imbei.uni-mainz.de:3838/iSEE
  • https://marionilab.cruk.cam.ac.uk/iSEE_allen
  • https://marionilab.cruk.cam.ac.uk/iSEE_tcga
  • https://marionilab.cruk.cam.ac.uk/iSEE_pbmc4k
  • https://marionilab.cruk.cam.ac.uk/iSEE_cytof

Please keep in mind that those public instances are for trial purposes only; yet they demonstrate how you or your system administrator can setup iSEE for analyzing or sharing your precomputed

SummarizedExperiment
/
SingleCellExperiment
object.

Extending iSEE

If you want to extend the functionality of iSEE, you can create custom panels which add new possibilities to interact with your data. Custom panels can be defined in independent R packages that include iSEE in the

Imports:
sections of their DESCRIPTION file. You can find a collection of working examples of how to do it in iSEEu. Feel free to contact the developing team, should you need some clarifications on how iSEE works internally.

Code of Conduct

Please note that the iSEE project is released with a Contributor Code of Conduct. By contributing to this project, you agree to abide by its terms.

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